Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid.

Using Saliva and Cerebrospinal Fluid Intravitam Diagnosis of Human Rabies by PCR

Real-time Sybr Green Pcr for Dog Rabies Diagnosis

The use of a 10-day observation to determine whether a dog is rabid is standard practice. This study was conducted in order to look for evidence of rabies vius in saliva and cerebrospinal fluid (CSF) of suspected live rabid dogs at the time of quarantine by using a SYBR Green real-time RT-PCR based assay for the detection of rabies virus RNA. Saliva and CSF of dogs were collected once on the da...

Real-time PCR analysis of dog cerebrospinal fluid and saliva samples for ante-mortem diagnosis of rabies.

The use of a 10-day observation to determine whether a dog is rabid is standard practice. This study was conducted in order to look for evidence of rabies vius in saliva and cerebrospinal fluid (CSF) of suspected live rabid dogs at the time of quarantine by using a SYBR Green real-time RT-PCR based assay for the detection of rabies virus RNA. Saliva and CSF of dogs were collected once on the da...

Imported Human Rabies, the Philippines and Finland, 2007

To the Editor: Rabies is a fatal zoonotic infection of the central nervous system caused by viruses of the genus Lyssavirus (1). Because person-to-person transmission is rare (2), public health action requirements in the countries where imported human rabies is seen are limited. However , communication with persons in places of exposure to provide information that could lead to public health ac...

Role of reverse transcriptase polymerase chain reaction for the diagnosis of human rabies

BACKGROUND & OBJECTIVES Traditionally, rabies diagnosis is made by demonstration of rabies viral antigen by direct immunofluorescence (DIF) and mouse inoculation test (MIT). The present study was carried out to evaluate the role of reverse transcriptase-polymerase chain reaction (RT-PCR) in comparison with these conventional techniques for the diagnosis of rabies. METHODS Skin biopsies, corne...